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KMID : 0869219970010010081
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Journal of Korean Geriatric Psychiatry
1997 Volume.1 No. 1 p.81 ~ p.95
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Effect of Ethanol on Short-term Memory in Rats : A Mechanism of Alcoholic Dementia
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Cheon Jin-Sook
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Abstract
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Chronic and excessive alcohol consumption produces a subtle brain damage, which induces such organic mental disorders as delayed mental processes, abstract thinking impairment, and disturbances in learning and recent memory loss. These phenomena had been known to be caused by malnutrition. However, recent researches showed that it
could be caused by mild brain lesions by direct neurotoxic effect of alcohol on the prefrontal cortex, or its related subcortical structures. This study was tried to evaluate the effect of alcohol on the short-term memory function, to compare with the histological changes, and to find out responses to the agonists and antagonists of possibly related neurotransmitters. For experiment 1, 10 aged male Sprague-Dawley rats weighed about 400-500 gm were used. 45 younger adult male Sprague-Dawley rats weighed about 200-300 gm were used for experiment 2 and 3. Therefore, 55 rats were totally used. In experiment 1, T-maze test with 10 normal aged rats were done first, and then it was divided into 5
atropine-administered group and 5 control group. For the atropine-administered group, T-maze test was repeated on every 30, 60, 120 minute artier the atropine injection. After the completion of behavioral tests, the rats were sacrificed by the intracardiac perfusion with phosphate buffered 10% formaldehyde solution, and the brawl specimen was stained with hematoxylin-rosin to count cells in the prefrontal cortex and the hippocampus. In experiment 2, T-maze test with 10 normal younger adult rats were done first, and then it was divided into five 14%(v/v) ethanol administered group and 5
control group raised with tap water. T-maze test was repeated on every week for a month. After the completion of behavioral tests on the 4th week, tustology was done by the same procedure. In experiment 3, T-maze test with 35 normal younger adult rats were done first, and then it was divided alto seven groups with five rats each other. 14%(v/v) ethanol was administered ad libitum. In addition, normal saline, fluoxetine, bromocriptine, bethacholine, nimodipine, clonidine, and ketamine were intramuscularly injected on every other day. T-maze test was repeated on every week for a month.
After the completion of behavioral tests on the 4th weed, histology was done by the same procedure. 1) The reaction time of T-maze test was more delayed on 120 minutes artier atropine injection in atropine-administered rats than those in normal aged rats without statistical significance. 2) The reaction time of T-maze test was more delayed in ethanol-treated rats, especially most prominent on the 3rd week, than those in normal younger adult rats without statistical significance. However, cell numbers in the CA1, CA3, dentate gyrus and the prefrontal cortex were significantly reduced in thanol-treated rats on histology (P<0.05). 3) The reaction time of T-maze test was more shortened in fluoxetine and
ketamine-treated rats on the 1st week without statistical significance. It was rather shortened in auoxetine, ketamine, bromocriptine and nimodipine-treated rats without statistical significance on the 2nd week. On the 3rd week the reaction time of T-maze test was shortened in every drug-treated rats. It returned to be delayed in all but fluoxetine, clonidine and bethacholine-treated rats on the 4th week without statistical significance. However, cell numbers in the CA1 were significantly increased in bromocriptine-treated rats (P<0.05) and in bethacholine-treated rats (P<0.01). In the CA3
and the dentate gyrus, cell numbers in bethacholine and clonidine-treated rats were significantly increased (P<0.05 respectively). In the prefiontal cortex, cell numbers in bethacholine-treated rats were significantly increased (P<0.005) on histology. 4) While there were no significant difference on the reaction time of T-maze test between ethanol-treated soup, normal aged group and atropine-treated group, cell numbers in the prefrontal cortex were significantly different between those of normal aged group and atropine-treated group (P<0.05). Cell numbers in the prefrontal cortex of ethanol-treated group were significantly correlated with those of atropine-treated group (r=0.977, P<0.001), and of normal aged group (r=0.448, P<0.05). In conclusion, we should not neglect the risk of memory loss even in the subclinical cases, because neuronal cells in the prefrontal cortex and the hippocampus were significantly reduced on histology, while ethanol-induced short-term memory loss was not functionally significant. The drug responses in this experiments showed that the
mechanism of alcohol-induced short-term memory loss might be mainly related with cholinergic system. Otherwise, adrenergic or dopaminergic mechanisms could be involved. Furthermore, we could not exclude the possibility that pathological aging process might be exerted as an important mechanism underlying alcoholic dementia.
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KEYWORD
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Short-term memory, T-maze, Hippocampus, Prefrontal cortex, Cholinergic, Catecholaminergic, Pathological aging.
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